Introduction
Exome sequencing is a technique for sequencing all the protein-coding genes in a genome (known as the exome). It consists of first selecting only the subset of DNA that encodes proteins (known as exons) and then sequencing that DNA using any high-throughput DNA sequencing technology. Quick Biology is a professional Whole Exome Seq (WES) service provider.
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Quick Biology WES Data Analysis Workflow:
Sample requirements:
DNA amount quantified by Nanodrop: ≥500 ng for normal input; minimum 20 ng
DNA concentration: ≥2 ng/μL
Total volume: ≥10 μL
Purity: OD260/280= 1.8-2.0 without degradation or RNA contamination
Bioinformatics service for WES:
a.Germline variants, compared to reference genome
b.Somatic mutations if Tumor-Normal pair (SNVs, InDel, CNV)
Turnaround Time
About 3-4 weeks, depends on the quality of submitted samples