A.
We will perform very strict quality control at every step from sample preparation to data analysis.
For wet lab part, we will check RNA/DNA sample concentration using Nanodrop and integrity using Bioanalyzer/Tapestation. Then after library construction, we will check the concentration of the library using Qubit and size of the library using Bioanalyzer/Tapestation. For data analysis part, we will check the quality of the raw FASTQ file, remove adaptor and perform quality trimming if necessary. Then we will provide the detailed alignment statistics including mappability, reads distribution in rRNA, intron, exon and intergenic region for RNA_seq. Exome coverage and duplication level will be provided for exome seq. All quality control documents will be sent to our customer. We know the good quality control is the key to a successful project.
